Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Chemotaxis Movement and Attachment of Agrobacterium Tumefaciens to Phalaenopsis Violacea Orchid Tissues: an Assessment of Early Factors Influencing the Efficiency of Gene Transfer

Subramaniam, S., and Balasubramaniam, V., and Poobathy, R., and Sreenivasan, S. , and Rathinam, X., (2009) Chemotaxis Movement and Attachment of Agrobacterium Tumefaciens to Phalaenopsis Violacea Orchid Tissues: an Assessment of Early Factors Influencing the Efficiency of Gene Transfer. Tropical Life Sciences Research, 20 (1). pp. 39-49. ISSN 1985-8345

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
166Kb

Official URL: http://www.usm.my/bio/bioscience/2009-20-01_files/TLSR%2020-1-5-SREERAMANAN%20%2839-49%29.pdf

Affiliations

Universiti Sains Malaysia, School of Biological Sciences.
AIMST University, Malaysia, Dept. of Biotechnology
AIMST University, Malaysia, Dept. of Biotechnology
Universiti Sains Malaysia. Institute for Research in Molecular Medicine
AIMST University, Malaysia, Dept. of Biotechnology

Abstract

An early step in the Agrobacterium-mediated transformation of Phalaenopsis violacea orchid was investigated to elucidate the plant-bacterium interaction. Directed movement in response to chemical attractants is of crucial importance to Agrobacterium tumefaciens strains. Chemotaxis of A. tumefaciens strains (EHA 101 and 105) towards wounded orchid tissues has been studied by using swarm agar plates. The results obtained indicate a minor role for chemotaxis in determining host specificity and suggest that it could not be responsible for the absence of tumourigenesis in P. violacea orchid under natural conditions. The spectrometric GUS and green fluorescent protein (GFP) assays provided information on the amount of inoculated A. tumefaciens that effectively bound to various orchid tissues. It can be concluded that, at least during the two early steps of interaction, A. tumefaciens appears to be compatible with P. violacea, indicating a potential basis for genetic transformation.

Item Type:Journal
Additional Information:The authors wish to thank Dr. Richard I.S. Bretell from CSIRO, Australia for the pCAMBIA 1304 plasmid. This research was supported financially by the Malaysian Science and Technology Toray Foundation (2005).
Keywords:Phalaenopsis violacea, Agrobacterium tumefaciens, Chemotaxis, Bacterial Attachment
Subjects:Q Science, Computer Science
ID Code:6592

Cheng M, Fry J E, Pang S, Zhou H, Hironaka C M, Duncan D R, Conner T W, and Wan, Y. (1997). Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiology 115: 971–980.

Chin D P, Mishiba K and Mii M. (2007). Agrobacterium-mediated transformation of protocorm-like bodies in Cymbidium. Plant Cell Reports 26: 735–743.

Gurlitz R H G, Lamb P W and Matthysse A G. (1987). Involvement of carrot surface proteins in attachment of Agrobacterium tumefaciens. Plant Physiology 83:564–68.

Hawes M C, Smith L Y and Howarth A J. (1988). Agrobacterium tumefaciens mutants deficient in chemotaxis to root exudates. Molecular Plant-Microbe Interactions 1: 182–186.

Hiei Y, Komari T and Kubo T. (1997). Transformation of rice mediated by Agrobacterium tumefaciens. Plant Molecular Biology 35: 205–218.

Kado C I. (1998). Agrobacterium-mediated horizontal gene transfer. Genetic Engineering 20: 1–24.

Lee Y W, Jin S G, Sim W S and Nester E W. (1996). The sensing of plant signal molecules by Agrobacterium: Genetic evidence for direct recognition of phenolic inducers by the VirA protein. Gene 179: 83–88.

Liau C H, You S J, Prasad V, Hsiao H H, Lu J C, Yang N S and Chan M T. (2003a). Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid. Plant Cell Reports 21: 993–998.

Mao H, Cremer P S and Manson D. (2003). A sensitive, versatile microfluidic assay for bacterial chemotaxis. Proceedings of the National Academy of Sciences. 100(9): 5449–5456.

Murashige T and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant 15: 473–497.

Perez Hernandez J B, Remy S, Galan Sauco V, Swennen R and Sagi L. (1999a). Chemotactic movement and attachment of Agrobacterium tumefaciens to banana cells and tissues. Journal of Plant Physiology 155: 245–250.

Remans T, Schenk P M, Manners J M, Grof C P C and Elliot A R. (1999). A protocol for the fluorometric quantification of mGFPS-ER and SGFP (S65T) in transgenic plants. Plant Molecular Biology Reporter 17: 385–395.

Shaw C H. (1991). Swimming against the side-chemotaxis in Agrobacterium Bioessays. 13: 25–29.

Shaw C H. (1995). Agrobacterium tumefaciens chemotaxis protocols. In: K M A Gartland and M R Davey (Ed.). Methods in Molecular Biology. Vol 44. Agrobacterium Protocols. 29–36.

Smith V A and Hindley J. (1978). Effect of agrocin 84 on attachment of Agrobacterium tumefaciens to cultured tobacco cells. Nature 276: 498–500.

Vergauwe A, Van Geldre W, Inze D, Van Montagu M and Van den Eeckhout E. (1998). Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. Plant Cell Reports 18: 105–110.

Wilson K J, Hughes S G and Jefferson R A. (1992). The Escherichia coli gus operon: Induction and expression of the gus operon in E. coli and the occurrence and use of GUS in other bacteria. In: S.R Gallagher (Ed.). GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression. 7–22.

53

Wright E L, Deakin W J and Shaw C H. (1998). A chemotaxis cluster from Agrobacterium tumefaciens. Gene 220: 83–89.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.