Inducible Transgene Expression in Undifferentiated Mouse Embryonic Stem Cells and Embryoid Bodies

Nordin N., and Nathan, S., and Li, M., and Mason, J.O., (2008) Inducible Transgene Expression in Undifferentiated Mouse Embryonic Stem Cells and Embryoid Bodies. Medical Journal of Malaysia, 63 (Supplement A). pp. 59-60. ISSN 0300-5283

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Affiliations

Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Dept. of Obstetrics and Gynaecology. Clinical Genetics Unit
Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Dept. of Obstetrics and Gynaecology. Clinical Genetics Unit
University of Edinburgh, UK. Institute for Stem Cell Research.
University of Edinburgh, UK. School of Biomedical Sciences

Abstract

Obtaining pure population of neural cells from embryonic stem (ES) cells remains a challenge as little is known about the genes that govern embryonic stem cell differentiation. Using mouse ES cells, we aim to uncover the mechanisms that regulate neural differentiation of ES cells by focusing on roles played by Wnt family genes. Combining two techniques, Cre/IoxP-based genetic recombination and ligand-dependent activation of Cre, we have generated transgenic ES cell lines that allow for the temporal control of expression and activity of Wnt gene (Wnt1-Ha) and Wnt antagonist (Dkk1). The ability of these cell lines in inducing the expression of transgene in undifferentiated ES cells and, more importantly, in differentiated derivatives of ES cells in vitro is evaluated.

Item Type:

Journal

Additional Information:

Using this system, expression of Wnt1-HA and Dkk1 from respective transgenic ES cell lines have been achieved in response to a nondetrimental dose of the synthetic oestrogen, 4’-OHT in undifferentiated ES cells as well as their differentiated derivatives in vitro. The cell lines will be very useful for inducing ectopic expression of the genes at any specific time point during neural differentiation assay in vitro, thus allowing us to examine, particularly, the consequences of overexpressing Wnt genes during the process.

Keywords:

Embryonic stem cells

Subjects:

R Medicine, Dentistry, Pharmacy, Nursing

ID Code:

6631

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