Influence of cryoprotectants on abnormality and motility of baung(Mystus nemurus) spermatozoa after long-term cryopreservation
Muchlisin Z. Abidin, and Siti Aziziah M. Nor, (2009) Influence of cryoprotectants on abnormality and motility of baung(Mystus nemurus) spermatozoa after long-term cryopreservation. Cryobiology, 58 . pp. 166-169.
Syiah Kuala University Banda Aceh Indonesia
Universiti Sains Malaysia Penang
Study on the effect of cryoprotectants on abnormality and motility of baung, Mystus nemurus spermatozoa were evaluated using transmission and scanning electron microscopy. Four cryoprotectants, dmso, ethanol, methanol and glycerol at concentration of 10% were tested in triplicates. Three ml of fresh sperm which was diluted with 60 ml of ringer solution was added to each of twelve 5-ml vials containing of 0.50-ml of the cryoprotectants. The vials were placed in an icebox containing dry ice 5 min and then storage into container containing liquid nitrogen for 13 months. The effect of cryoprotectants on the spermatozoa abnormality and motility were significant (P < 0.05). The spermatozoa abnormality was significantly lower in methanol (62.65%) compared with the other cryoprotectants. The spermatozoa motility was higher in methanol, but not significantly different with ethanol (P > 0.05). It is a negative correlation between sperm motility and abnormality. Generally, higher abnormalities of spermatozoa resulted low motility.
|Additional Information:||We express appreciate to Prof. Dr. Roshada Hashim for her
suggestion. The technical assistance by Mr. Pancamuthu Ramasamy and all members of aquaculture research group of University Sains Malaysia and Syiah Kuala University are also acknowledged.|
|Keywords:||Baung Mystus nemurus, Cryoprotectant,Spermatozoa, Abnormality and motility|
|Subjects:||S Agriculture, Forestry|
Q Science, Computer Science
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