Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Influence of cryoprotectants on abnormality and motility of baung(Mystus nemurus) spermatozoa after long-term cryopreservation

Muchlisin Z. Abidin, and Siti Aziziah M. Nor, (2009) Influence of cryoprotectants on abnormality and motility of baung(Mystus nemurus) spermatozoa after long-term cryopreservation. Cryobiology, 58 . pp. 166-169.

[img]PDF - Registered users only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
496Kb

Affiliations

Syiah Kuala University Banda Aceh Indonesia
Universiti Sains Malaysia Penang

Abstract

Study on the effect of cryoprotectants on abnormality and motility of baung, Mystus nemurus spermatozoa were evaluated using transmission and scanning electron microscopy. Four cryoprotectants, dmso, ethanol, methanol and glycerol at concentration of 10% were tested in triplicates. Three ml of fresh sperm which was diluted with 60 ml of ringer solution was added to each of twelve 5-ml vials containing of 0.50-ml of the cryoprotectants. The vials were placed in an icebox containing dry ice 5 min and then storage into container containing liquid nitrogen for 13 months. The effect of cryoprotectants on the spermatozoa abnormality and motility were significant (P < 0.05). The spermatozoa abnormality was significantly lower in methanol (62.65%) compared with the other cryoprotectants. The spermatozoa motility was higher in methanol, but not significantly different with ethanol (P > 0.05). It is a negative correlation between sperm motility and abnormality. Generally, higher abnormalities of spermatozoa resulted low motility.

Item Type:Journal
Additional Information:We express appreciate to Prof. Dr. Roshada Hashim for her suggestion. The technical assistance by Mr. Pancamuthu Ramasamy and all members of aquaculture research group of University Sains Malaysia and Syiah Kuala University are also acknowledged.
Keywords:Baung Mystus nemurus, Cryoprotectant,Spermatozoa, Abnormality and motility
Subjects:S Agriculture, Forestry
Q Science, Computer Science
ID Code:6722

[1] M.M. Adam, K.J. Rana, B.J. McAndrew, Effect of cryoprotectants on activity of selected enzymes in fish embryos, Cryobiology 32 (1995) 92–104.

[2] A. Bart, New approaches in cryopreservation of fish embryos, in: T.R. Tiersch, P.M. Mazik (Eds.), Cryopreservation in Aquatic Species, World Aquaculture

Society, Baton Rouge, Louisiana, 2000, pp. 179–187.

[3] N.H. Chao, I.C. Liao, Cryopreservation of finfish and shellfish gametes and embryos, Aquaculture 197 (2001) 161–189.

[4] N.H. Chao, Fish sperm cryopreservation in Taiwan: Technology advancement and extension efforts. Paper on International Symposium on Reproductive Biology in Aquaculture. Dept. of Aquaculture, Taiwan Fisheries Res. Ins., Taiwan, 1991, pp. 31.

[5] N.H. Chao, C.P. Chiang, H.C. Hsu, C.T. Tsai, T.T. Lin, Toxicity tolerance of oyster embryos to selected cryoprotectants, Aquatic Living Resources 7 (1994) 99–104.

[6] J.R. Correa, P.M. Zavos, Preparation and recovery of frozen-thawed bovine spermatozoa via various sperm selection techniques employed in assisted reproductive technologies, Theriogenology 46 (1996) 1225–1232.

[7] J.R. Correa, G. Heersche Jr., P.M. Zavos, Sperm membrane functional integrity and response of frozen-thawed bovine spermatozoa during the hypoosmotic swelling test incubation at varying temperatures, Theriogenology 47 (1997)

715–721.

[8] S. Dronkin, H. Stein, H. Bartcherer, Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta F. fario), Cryobiology 37 (1998) 263–270.

[9] G.M. Fahy, The relevance of cryoprotectant ‘toxicity’ to cryobiology, Cryobiology 23 (1986) 1–13.

[10] T. Farkas, E. Fodor, K. Kitajka, J.E. Halver, Response of fish membranes to environmental temperature, Aquaculture Research 32 (2001) 645–655.

[11] J.C. Gwo, C.R. Arnold, Cryopreservation of Atlantic croaker spermatozoa: evaluation of morphological changes, Experimental Zoology 264 (1992) 44–453.

[12] B. Harvey, M.J. Ashwood-Smith, Cryoprotectant penetration and supercooling in the eggs of salmonid fish, Cryobiology 19 (1982) 29–40.

[13] F. Lahnsteiner, T. Weismann, R.A. Patzner, Fine

structure changes in spermatozoa of the grayling, Thymallus thymallus (Pisces: Teleostei), during routine cryopreservation, Aquaculture 103 (1992) 73–84.

[14] F. Lahnsteiner, T. Weismann, R.A. Patzner, Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of semen from salmonid fishes, Aquaculture Research 28 (1997) 471–479.

[15] F. Lahnsteiner, N. Manour, T. Weismann, A new technique for insemination of large egg batches with cryopreserved semen in the rainbow trout, Aquaculture 209 (2002) 359–367.

[16] X.H. Liu, T. Zhang, D.M. Rawson, Effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (Danio rerio) embryos, Theriogenology 55 (2001) 1719–1731.

[17] Z.A. Muchlisin, R. Hashim, A.S.C. Chong, Preliminary study on the cryopreservation of tropical bagrid catfish (Mystus nemurus) spermatozoa; the effect of extender and cryoprotectant on the motility after short-term storage, Theriogenology 62 (2004) 25–34.

[18] Z.A. Muchlisin, Review: Current status of extenders and cryoprotectants on fish spermatozoa cryopreservation, Biodiversitas 6 (2005) 12–15.

[19] B. Ogier de Baulny, C. Labbe, G. Maisse, Membrane integrity, mitochondrial activity, ATP content, and motility of the European catfish (Silarus glanis) testicular spermatozoa after freezing with different cryoprotectant, Cryobiology 39 (1999) 177–184.

[20] H. Ohta, K. Kawamura, T. Unuma, Y. Takegoshi, Cryopreservation of the sperm of the Japanese bitterling, Journal of Fish Biology 58 (2001) 670–681.

[21] K. Rana, Preservation of gametes, in: N.R. Bromage, R.J. Roberts (Eds.), Broodstock Management and Egg and Larval Quality, University Press Cambridge, England, 1995, pp. 53–75.

[22] M. Shafer, Pharmacological considerations in cryopreservation, in: A.M. Karow, D.E. Pegg (Eds.), Organ Prevention of Transplantation, 1981, pp. 177–212.

[23] F.P. Simione, Cryopreservation manual, Nalge Nunc Inter. Corp., New York, 1998. 8.

[24] A.R. Taddei, F. Barbato, L. Abelli, S. Canese, F. Moretti, K.J. Rana, A.M. Fausto, M. Mazzini, Is cryopreservation a homogenous process? Ultrastructure and motility of untreated, prefreezing, and postthawed spermatozoa of Diplodus puntazzo Cetti, Cryobiology 42 (2001) 244–255.

[25] A.T.M. Viveiros, N. So, J. Komen, Sperm cryopreservation of African catfish, Clarias gariepinus: cryoprotectants, freezing rates and sperm: egg dilution ratio, Theriogeneology 54 (2000) 1395–1408.

[26] M. Wusteman, U. Rauen, J. Simmonds, N. Hunds, D.E. Pegg, Reduction of cryoprotectant toxicity in cell in suspension by use a sodium-free vehicle solution, Cryobiology 56 (2008) 72–79.

[27] J.H. Zar, Biostatistical analysis, 2nd edition., Prentice Hall, Englewood, USA, 1984.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.