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Cytotoxicity Against Tumor Cell Lines Of A Ribosomeinactivating Protein (Rip)-Like Protein Isolated From Leaves Of Mirabilis Jalapa L.

Ikawatip, Zullies, and Sudjadp, and Sismindari, (2006) Cytotoxicity Against Tumor Cell Lines Of A Ribosomeinactivating Protein (Rip)-Like Protein Isolated From Leaves Of Mirabilis Jalapa L. Malaysian Journal of Pharmaceutical Sciences, 4 (1). pp. 31-41. ISSN 1675-7319

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Official URL: http://www.pha.usm.my/pharmacy/Webpage%20journal/abstract/4.1-4.pdf

Affiliations

Gadjah Mada University, Faculty of Pharmacy, Indonesia.
Gadjah Mada University, Faculty of Pharmacy, Indonesia.
Gadjah Mada University, Faculty of Pharmacy, Life Science Laboratory, Indonesia.

Abstract

The 30 kD protein fraction with properties like ribosome-inactivating protein (RIP) was isolated from the leaves of Mirabilis jalapa L. and named MI-30. This study investigated the cytotoxic effect of MI-30 on normal and malignant cells. MI-30 was isolated from the leave extract of M. jalapa L. using cation-exchange chromatography with CM Sepharose CL-6B column to obtain MI-30. The fraction contain DNA cleaving ability were pooled and checked for cytotoxicity against breast cancer T47D cell line, cervical cancer SiHa cell line, and human mononuclear cells derived from peripheral blood of healthy volunteers: Results showed that the MI-30 produced cytotoxic effect against T47D and SiHa cell line to different extent. The LCso of the MI-30 on T47D cell line and SiHa cell line were 0.36 pg/mL and 5.6 pg/mL, respectively. While in normal cells, represented by human mononuclear cells, MI-30 was considerably less toxic, with LC50 of 21.04 pg/mL. The results suggest that MI-30 produced more cytotoxic activity toward breast and cervical cancer cells (58-fold and 4-fold, respectively) as compared to normal mononuclear cells.

Item Type:Journal
Additional Information:This work was supported by grants from Quality of Undergraduate Education (QUE) Program Batch III, Faculty of Pharmacy, Gadjah Mada University. We would like to thank Ms. Leny Wirawan, Helena Siboro, and Ni Kadek Darsini for their technical assistance.
Keywords:Ribosome-inactivating protein, Mirabilis jalapa L., Cytotoxicity, T47D and SiHa cell line, Human mononuclear cells
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:6813

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