Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

The Antiproliferative Effects Of Zingiber Zerumbet Extracts And Fractions On The Growth Of Human Breast Carcinoma Cell Lines

Rumiza Abd Rashid, and Azimahtol Hawariah Lope Pihie, (2005) The Antiproliferative Effects Of Zingiber Zerumbet Extracts And Fractions On The Growth Of Human Breast Carcinoma Cell Lines. Malaysian Journal of Pharmaceutical Sciences, 3 (1). pp. 45-52. ISSN 1675-7319

[img]PDF - Registered users only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
190Kb

Official URL: http://www.pha.usm.my/pharmacy/Webpage%20journal/abstract/3.1-5.pdf

Affiliations

Malaysian Agricultural Research and Development Institute
Universiti Kebangsaan Malaysia, Faculty of Science and Technology, School of Bioscience and Biotechnology

Abstract

Zingiber zerumbet, from Zingiberaceae family, is known for its medicinal properties. From the ethnopharmacology and laboratory studies, extracts and active component of this plant, zerumbone, were reported to have the antiinflammatory, antioxidant, antimicrobial and antitumour properties. However, no studies have been done to verify the antiproliferative activity of this plant extracts on
breast cancer cells. The present study investigated the effects of extracts and fractions of Z. zerumbet on the growth of human breast carcinoma (MCF-7) cell lines. The number of living cells was evaluated by methylene blue staining technique. Our data indicated that Z. zerumbet
extracts and some fractions exhibited antiproliferative effect on MCF-7. Bioassay-guided fractionation of petroleum ether extract, the most active crude extract, with EC 50 of 4.25 ± 0.05 gg/mL resulted in the isolation of two active subfractions. One of them was found to contain 2,6,10-cycloundecatrien-1-one, 2,6,9,9,-tetramethyl with 94% similarity to zerumbone obtained from the GC-MS analysis.
A comparative study between both subfractions and tamoxifen showed that the active subfractions of Z. zerumbet displayed a strong antiproliferative effect with ECso of
2.81 ± 0.24 and 2.49 ± 0.13 pg/mL, respectively, on MCF-7 but less cytotoxic to normal cells.

Item Type:Journal
Additional Information:We would like to express our gratitude to Mr Suri Roowi from MARDI, Serdang, Selangor for the GC-MS data. Financial support of this study was provided by the Ministry of Science, Technology and Environment through IRPA 06-02-02-001 BTK/ ER/ 015(3a).
Keywords:Zingiber zerumbet, zerumbone, bioassay-guided fractionation, cell proliferation assay, ginger, plants, medicinal properties
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:6831

DAMODARAN, N.P. & DEV, S. (1%8) Studies in sesquiterpenes - XXXVII.Sesquiterpenoids from the essential oil of Zingiber zerumbet Smith, Tetrahedron, 24: 4113-4122.

GEBHARDT, R. (2000) In vitro screening of plant extracts and phytopharmaceuticals: Novel approaches for the elucidation of active compounds and their mechanisms, Planta

Medico, 66: 99-105

HOFFMAN, A., SPETNER, L.M. & BURKE, M. (2002) Redox-regulated mechanism may account for zerumbone's ability to suppress cancer-cell proliferation, Carcinogenesis, 23: 1961.

KINGHORN, A.D., FARNSWORTH, N.R., SOEJARTO, D.D., CORDELL, GA., PEZZUTO, J.M., UDEANI, G.O., WANI, M.C., WALL, M.E., NAVARRO, H.A., KRAMER, R.A., MENENDEZ, A.T., FAIRCHILD, C., LANE, K.E., FORENZA, S., WAS, D.M., LAM, K.S. & SHU, Y.Z. (1999) Novel strategies for the discovery of plant-derived anticancer agents, Pure Applied Chemistry, 71: 1611-1618.

LIN, L. & HVVANG, P. (1991) Antiproliferative effects of oxygenated sterols: Positive correlation with binding affinities for the antiestrogen-binding sites, Biochimica et

Biophysica Acta., 1082: 177-184.

MATTHES, H.W.D., LUU, B. & OURISSON, G. (1980) Cytotoxic components of Zingiber zerumbet, Curcuma zedoria and Curcuma domestica, Phytochemistry, 19: 2643-2650.

MURAKAMI, A., TAKAHASHI, D., KINOSHITA, T., KOSHIMIZU, K., HA, W.K., YOSHIHIRO, A., NAKAMURA, Y., JIWAJINDA, S., TERAO, J. & OHIGASHI, H. (2002). Zerumbone, a Southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: The a, n-unsaturated carbonyl group is a prerequisite, Carcinogenesis, 23: 759-802.

PERRY, L.M. & METZGER, J. (1980) Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses, pp. 444 (Cambridge, MA: MIT Press).

SRIVASTARA, A.K., SRIVASTARA, S.K. & SHAH, N.C. (2000) Essential oil composition of Zingiber zerumbet (L) Sm from India, Journal of Essential Oil Research,12: 595-597.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.