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Scanning Electron Microscopy of Cornea Re-Epithelization After Transplanted with Bilayered Corneal Construct

Masrudin S.S., and Ghafar N.A., and Saidi M., and Aminuddin B.S., and Rahmat A., and Ruszymah B.H.I., and Othman F., (2008) Scanning Electron Microscopy of Cornea Re-Epithelization After Transplanted with Bilayered Corneal Construct. Medical Journal of Malaysia, 63 (Supplement A). pp. 109-110. ISSN 0300-5283

Full text not available from this repository.

Affiliations

Universiti Putra Malaysia. Faculty of Medicine and Health Science. Dept. of Human Anatomy.
Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Anotomy.
Universiti Putra Malaysia. Faculty of Medicine and Health Science. Dept. of Biomedicine.
Ampang Puteri Specialist Hospital. Ear, Nose and Throat Consultant Clinic.
Universiti Putra Malaysia. Faculty of Medicine and Health Science. Dept. of Nutrition and Dietetic.
University Kebangsaan Malaysia. Faculty of Medicine. Dept. of Physiology.
Universiti Putra Malaysia. Faculty of Medicine and Health Science. Dept. of Human Anatomy.

Abstract

The present work was to determine the development and re-epithelization of bilayered corneal construct (BCC) in vitro and in vivo using scanning electron microscopy (SEM). The in vitro BCC was transplanted to the rabbit’s eye and after 90 days the BCC was harvested and analyzed. The corneas were processed for morphology studies. The result indicates that the BICC that was transplanted for 90 days showed good development and re-epithelization of epithelial layer similar to the normal cornea.

Item Type:Journal
Additional Information:In conclusion, our data indicated that BCC after 90 days post- transplantation demonstrate evidence of re-epithelization. This finding suggests that BCC can be a corneal substitute for the cornea disorder patients in future.
Keywords:bilayered corneal construct, Opthamology
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:7926

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4. Tsubota K, et al. Treatment of severe ocular surface disorders with corneal epithelial stem cell transplantation. N Engl J Med. 1999; 340: 1697-703.

5. Schneider AI, Maier-Reif K and Graeve T. Constructing an in vitro cornea of the specific corneal cell types. In vitro Cellular and Developmental Biology. 1999;35(9):515-26.

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