Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Diagnosis of Acute Diarrhea in Pigs and Piglets in Meghalaya, India

Das, A., and Mazumder, Y., and Dutta. B.K., and Kumar, A., and Selvi, S., (2009) Diagnosis of Acute Diarrhea in Pigs and Piglets in Meghalaya, India. Malaysian Journal of Microbiology , 5 (1). pp. 38-44. ISSN 9834240708

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
288Kb

Official URL: http://www.usm.my/mjm/issues/vol5no1/research7.pdf

Affiliations

Bannari Amman Institute of Technology, India. Dept. of Biotechnology
Nagarjuna College of Engineering and Technology, India, Dept. of Biotechnology Engineering
Assam University, India, Dept. of Ecology and Environmental Science
Indian Council of Agricultural Research for NEH Region, Division of Animal Health
Bannari Amman Institute of Technology, India, Dept. of Biotechnology

Abstract

This paper reports the diagnosis of acute diarrhoea in three male pigs and two female piglets died in an organized pig farm in Meghalaya, India. Intestinal contents and liver samples from dead pigs and piglets were investigated for isolation and identification of bacteria. Bacterial isolation confirmed the causative agent as Clostrodium perfringens. A total of eight C. perfringens were isolated out of 10 clinical samples. Isolates harbored identical double plasmid DNA of molecular weights 42.8 kb and 51.9 kb common to all. In polymerase chain reaction (PCR), all the eight (100%) clinical isolates harboured alpha toxin gene (cpa) and beta2 toxin gene (cpb2). None of the isolates were positive for beta, epsilon, iota and enterotoxin genes. The PCR analysis revealed that all isolates derived from acute diarrhoea belonged to C. perfringens type A. The partial cpa gene sequences from two Indian field isolates in BLAST showed similarity values greater than 99% to the published database sequences of C. perfringens. The phylogenetic analysis also revealed 98.6 to 100% sequence homology among the isolates studied. The findings suggested that C. perfringens type A having additional cpb2 gene might play a significant role in occurrence of acute diarrhoea in pigs and piglets in Meghalaya, India.

Item Type:Journal
Keywords:Clostridium perfringens, acute diarrhoea, pig, piglet, plasmids, polymerase chain reaction, swine
Subjects:Q Science, Computer Science
ID Code:8339

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology 215, 403-410.

2. Birnboim, H. C. and Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Research 7, 1513-1523.

3. Bueschel, D. M., Jost, B. H., Billington, S. J., Trinh, H. T. and Songer, J. G. (2003). Prevalence of cpb2, encoding beta2 toxin, in C. perfringens field isolates: correlation of genotype with phenotype. Veterinary Microbiology 94, 121-129.

4. Czanderlova, L., Hlozek, P., Chmelar, D. and Lany, P. (2006). Clostridium perfringens in suckling piglets with diarrhea and its PCR typing and prevalence in the Czech Republic in 2001–2003. Veterinarni Medicina 51, 461-467.

5. Czeczulin, J. R., Hanna, P. C. and Mcclane, B. A. (1993). Cloning, nucleotide sequencing, and expression of the C. perfringens enterotoxin gene in Escherichia coli. Infection and Immunity 61, 3429-3439.

6. Eisgruber, H., Wiedmann, M. and Stolle, A. (1996). Plasmid profiling for strain differentiation and characterization of C. perfringens isolates. Zentralbl Veterinarmed B 43, 137-146.

7. Garmory, H. S., Chanter, N., French, N. P., Bueschel, D., Songer, J. G. and Titball, R. W. (2000). Occurrence of C. perfringens β2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiology and Infection 124, 61-67.

8. Gibert, M., Jolivet, R. C. and Popoff, M. R. (1997). Beta2 toxin, a novel toxin produced by C. perfringens. Gene 203, 65-73.

9. Ginter, A., Williamsons, E. D., Dessy, F., Coppe, P., Bullifent, H., Howells, A. and Titball, R. (1996). Molecular variation between the alpha toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animals. Microbiology 142, 191-198.

10. Holt, J. G., Krige, N. R., Sneeth, P. H. A., Staley, J. T. and Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology, 9th ed. Williams and Wilkins, Baltimore, USA, pp, 749-755.

11. Hunter, S. E. C., Clarke, I. N., Kelly, D. C. and Titball, R. W. (1992). Cloning and nucleotide sequencing of the C. perfringens epsilon-toxin gene and its expression in Escherichia coli. Infection and Immunity 60, 102-110.

12. Hunter, S. E. C., Brown, J. E., Oyston, P. C., Sakurai, J. and Titball, R. W. (1993). Molecular genetic analysis of beta-toxin of C. perfringens reveals sequence homology with alpha-toxin, Gamma-toxin, and leukocidin of Staphylococcus aureus. Infection and Immunity 61, 3958-3965.

13. Kanakaraj, R., Harris, D. L., Songer, J. G. and Bosworth, B. (1998). Multiplex PCR assay for detection of Clostridium perfringens in feces and intestinal contents of pigs and swine feed. Veterinary Microbiology 63, 29-38.

14. Klaasen, H. L., Molkenboer, M. J., Bakker, J., Miserez, R., Hani, H., Frey, J., Popoff M. R. and Van Den Bosch, J. F. (1999). Detection of the beta2 toxin gene of C. perfringens in diarrhoeic piglets in the Netherlands and Switzerland. FEMS Immunology and Medical Microbiology 24, 325-332.

15. Perelle, S., Gibert, M., Boquet, P. and Popoff, M. R. (1993). Characterization of C. perfringens iota-toxin genes and expression in Escherichia coli. Infection and Immunity 61, 5147-5156.

16. Rooney, A. P., Swezey, J. L., Friedman, R., Hecht, D. W. and Maddox, C. W. (2006). A analysis of core housekeeping and virulence genes reveals cryptic lineages of C. perfringens that are associated with distinct disease presentations. Genetics 172, 2081-2092.

17. Shome, B. R., Songer, J. G., Shakuntala, I., Shome, R., Kumar, A., Chakraborty, S., Mazumder, Y., Rahman, M. M., Das, A., Murugkar, H. V., Rahman, H. and Bujarbaruah, K. M. (2006). Atypical blackleg caused by C. perfringens type A in cattle in Manipur, India. Indian Journal of Animal Science 76, 353-357.

18. Songer, J. G and Meer, R. R. (1996). Genotyping of C. perfringens by polymerase chain reaction is a useful adjunct to diagnosis of clostridial enteric disease in animals. Anaerobe 2, 197-203.

19. Songer, J. G. (1996). Clostridial enteric diseases of domestic animals. Clinical Microbiology Review 9, 216-234.

20. Squires, C. H., Heefner, D. L., Evans, R. J., Kopp, B. J. and Yarus, M. J. (1984). Shuttle plasmids for Escherichia coli and C. perfringens. Journal of Bacteriology 159, 465-471.

21. Titball, R. W., Hunter, S. E. C., Martin, K. L., Morris, A. D., Shuttleworth, A. D., Rubdige, T., Anderson, D. W. and Kelly, D. C. (1989). Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of C. perfringens. Infection and Immunity 57, 367-376.

22. Waters, M. A. S., Garmory, H. S., Bueschel, D., Popoff, M. R., Songer, J. G., Titball, R. W., Mcclane, B. A. and Sarker, M. R. (2003). Genotyping and phenotyping of beta2-toxigenic Clostridium perfringens fecal isolates associated with gastrointestinal diseases in piglets. Journal of Clinical Microbiology 41, 3584-359.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.