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Activity Of Acid Phosphatase In The Ectomycorrhizal Fungus Cantharellus Tropicalis Under Controlled Conditions

Baghel, R.K., and Sharma, R., and Pandey, A.K., (2009) Activity Of Acid Phosphatase In The Ectomycorrhizal Fungus Cantharellus Tropicalis Under Controlled Conditions. Journal of Tropical Forest Science, 21 (3). pp. 218-222. ISSN 0128-1283

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Official URL: http://info.frim.gov.my/cfdocs/infocenter/jtfsonline/jtfs/v21n3/218-222.pdf

Affiliations

R.D. University, Dept. of Biological Sciences, Mycological Research Laboratory
R.D. University, Dept. of Biological Sciences, Mycological Research Laboratory
R.D. University, Dept. of Biological Sciences, Mycological Research Laboratory

Abstract

Ectomycorrhizal fungi benefit host plant by absorbing phosphorus with the help of acid and alkaline phosphatases. Acid and alkaline phosphatases are cell wall-bound enzymes. Cantharellus tropicalis were grown in axenic cultures to determine the activity of acid phosphatase by a quantitative colorimetric method. The effects of pH and temperature on the activity of surface acid phosphatase were also studied. Results showed that the optimal pH and temperature of the C. tropicalis strain were 4 and 40 oC respectively.

Kulat ektomikoriza memberi faedah kepada tumbuhan perumah kerana ia menyerap fosforus dengan bantuan asid fosfatase serta alkali fosfatase. Asid fosfatase serta alkali fosfatase merupakan enzim yang terikat pada dinding sel. Cantharellus tropicalis dibiakkan untuk menentukan aktiviti asid fosfatase menggunakan kaedah kolorimetri. Kesan pH dan suhu terhadap aktiviti asid fosfatase permukaan turut dikaji. Keputusan menunjukkan bahawa pH dan suhu optimum strain C. tropicalis adalah masing-masing 4 dan 40 oC.

Item Type:Journal
Additional Information:Financial assistance received from the Department of Biotechnology, New Delhi, India in the form of a major research project No: BT/ PR3916/PID/20/153/2003 is acknowledged. The authors are also thankful to the Head, Department of Biological Sciences, R.D. University for providing laboratory facilities.
Keywords:Alkaline phosphatase, cell wall-bound enzymes, p-NPP, Dendrocalamus strictus, pH, temperature, Fumgi
Subjects:S Agriculture, Forestry
ID Code:8544

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