creators_name: Siti Balkis Budin,  
creators_name: Nor Fadilah Rajab,  
creators_name: Khairol Osman,  
creators_name: Abdul Gapor Mat Top ,  
creators_name: Wan Nazaimoon Wan Mohamud,  
creators_name: Mokhtar Abu Bakar,  
creators_name: Jamaludin Mohamed,  
type: article
datestamp: 2008-07-01 14:47:02
lastmod: 2008-07-01 14:47:02
metadata_visibility: show
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Allied Health Sciences. Dept. of Biomedical
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Allied Health Sciences. Dept. of Biomedical
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Allied Health Sciences. Dept. of Biomedical
corp_creators: Malaysian Palm Oil Board
corp_creators: Institute for Medical Research Malaysia. 
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Pathology
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Allied Health Sciences. Dept. of Biomedical
title: Effects of Palm Vitamin E Against Oxidative Damage in Streptozotocin-induced Diabetic Rats
ispublished: pub
subjects: Q
subjects: R
full_text_status: none
keywords: Diabetes mellitus, palm vitamin E, antioxidant, oxidative Stress, DNA damage
abstract: Palm vitamin E, which rich in tocotrienol is a highly effective antioxidant and able to reduce oxidative stress in various pathological conditions. Diabetes mellitus is often associated with increase in the production of reactive oxygen species together with reduction in antioxidants levels. In order to elucidate the antioxidative effects of palm vitamin E, we investigated the effects of 8 weeks supplementation with palm vitamin E on glycaemic status, malondialdehyde levels and DNA damage in Streptozotocininduced diabetic rats. Alkaline Comet Assay assessed protective effects against DNA damage to peripheral blood. Streptozotocin diabetic rats were force fed daily with palm vitamin E (200mg/kg body weight), from day three of diabetes condition established. Following eight weeks of supplementation, palm vitamin E significantly improved fasting blood glucose and glycosylated hemoglobin. The increased in plasma malondialdehyde levels were also inhibited and the levels of oxidative DNA damage of peripheral lymphocyte was reduced. This suggested that palm vitamin E has the potential in improving glycaemic status and also the protective effects against oxidative damage by inhibiting the increased in malondialdehyde and preventing DNA damage in Streptozotocin-induced diabetic rats.
date: 2006
date_type: published
publication: Malaysian Journal of Biochemistry and Molecular Biology
volume: 13
number: 1
publisher: Malaysian Society for Biochemistry and Molecular Biology
pagerange: 11-17
refereed: TRUE
issn: ISSN 1511-2616
official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=587
referencetext: 1. Hunt JV, Dean RT, Wolff SP. Hydroxyl radical production and autoxidative glycosylation. Biochem. J. 1988; 256:205-212.

2. Lacka B, Grzeszcrzak W, Strojek K, Twardowska K, Froehlich J, Jendryczko A. Pro-oxidant-antioxidant imbalance in insulin dependent diabetes mellitus. Diabetologia. 1995; 38(Suppl.1):A40.

3. Hannon-Fletcher MAP, O’Kane MJ, Moles KW, Weatherup C, Barnett CR, Barnett YA. Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subject. Mutation Research. 2000; 460:53-60.

4. Draper HH. and Hadley M. Malondialdehyde determination as index of lipid peroxidation. Meth Enzymology. 1990;186:421-430.

5. Vaca CE, Wilhelm J, Harms-Ringdah M. Interaction of lipid peroxidation product with DNA. A review. Mutat. Res. 1988; 195: 137-149.

6. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehyde. Free Radical Biol. Med. 1991; 11:81-88.

7. Laight DW, Desai KM, Gopaul NK, Erik E Änggard EE, Carrier MJ. F2-isoprostane evidence of oxidant stress in the insulin resistant, obese Zucker rat: effects of vitamin E. European Journal of Pharmacology. 1999; 377: 89-92

8. Weber C, Podda M, Rallis M, Thiele JJ, Traber MG, Packer L. Efficacy of Topically Applied Tocopherols and Tocotrienols in Protection of Murine Skin From oxidative Damage Induced by UV-Irradiation. Free Radical Biology and Medicine. 1997; 22(5):761-769.

9. Abdul Mutalib M.S., Khaza’ai H, Wahle KWJ. Palmtocotrienol rich fraction (TRF) is a more effective inhibitor of LDL oxidation and endothelial cell lipid peroxidation than (-tocopherol in vitro. Food Research International.2003; 36(5): 405-413.

10. Musalmah M., Muhd Fairuz A.H., Gapor M.T., Wan Ngah W.Z. Effect of palm vitamin E on wound healing in streptozotocin-induced diabetic rats. Malaysian Journal of Biochemistry and Molecular Biology. 2001; 6: 34-39.

11. Eross J, Kreutzmann D, Jimenez M, Keen R, Roger S, Cowell C, Vines R, Silink M. Colometric measurement of glycosylated protein in whole blood, red blood cells, plasma and dried blood. Annal. Clinical Biochemistr. 1984; 21:477-483.

12. Bradford MM. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976; 72:248-254.

13. Sigh NP, McCoy T, Tiee RR, Schneider EL. A simple technique for measuring low levels of DNA damage in individual cells. Exp. Cell Res. 1988; 175:184-192.

14. Martin-Gallan P, Carracosa A, Gussinye M, Dominguez C. Biomarkers of diabetes-associated oxidative stress and antioxidant status in young diabetic patients with or without sub clinical complications. Free Rad. Bio. Med. 2003; 34(12): 1563-1574.

15. Seghrouchni I, Drai J, Bannier E, Riviere J, Calmard P, Garcia I, Orgiazzi J, Revol A. Oxidative stress in type 1, type 11, and insulin treatment efficiciency. Clin. Chim. Acta 2002; 321: 89-96.

16. Andallu B and Varadacharyulu NC. antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Clinica Chimica Acta. 2003; 338: 3-10.

17. Jakus V, Bauerova K, Michalkova D, Carsky J. Value markers of advanced glycation and lipoxidation in serum proteins of children with diabetes mellitus. Bratisl. Lek. Listy. 2000; 101 (9): 484-489.

18. Luostarinen R, Wallin R, Wibel L, Saldeen T. Vitamin E supplementation counteracts the fish oil-induced increase of blood glucose in humans. Nutrition Research. 1995;15(7): 953-968.

19. Theriault A, Chao JT, Wang Q, Gapor A, Adeli K. Tocotrienol: A Review of its therapeutic potential. Clinical Biochemistry. 1999; 32: 309-319.

20. Lanzen S, Drinkgern J, Tiedge M. Low antioxidant gene expression in pancreatic islets compared with various other mouse tissues. Free Radic. Biol. Med. 1996; 20:463-466.

21. Ihara Y, Yamada Y, Toyokuni S, Uchida K, Odaka H, Tanaka T, Ikeda H, Hiai H, Seino Y, Yamada Y. Hyperglycemia causes oxidative stress in pancreatic beta cells of GK rats, a model of type 2 diabetes. Diabetes. 1999; 48: 927-932

22. Kamal-Eldin A and Appelqvist LA. The chemistry and antioxidant properties of tocopherol and tocotrienols. Lipid. 1996; 31: 671-701.

23. Ihara Y, Yamada Y, Toyokuni S, Miyawaki K, Ban N, Adachi T, Kuroe A, Iwakura T, Kubota A, Hiai , Seino Y. Antioxidant _-tocopherol ameliorates glycemic control of GK rats, a model of type 2 diabetes. FESEB Letters. 2000; 474: 24-26.

24. Collins AR, Raslova K, Somoroska M, Petrovska H, Ondrusova A, Vonnout B, Fabry R, Dusinska M. DNA damage in diabetes: Correlation with a clinical marker. Free Rad. Biol. Med. 1998; 25(3): 373-377.

25. Lorenzi M, Montisano DF, Toledo S, Wong HC. Increased single strand breaks in DNA of lymphocyte from diabetic subjects, J. Clin. Invest. 1987; 79: 653-656.

26. Imaeda A, Kaneko T, Aoki T, Kondo Y, Nakamura N, Nagase H, Yoshikawa T. Antioxidative effect of fluvastatin and its metabolites against DNA damage in streptozotocintreated mice. Food and Chemical Toxicology. 2002;40:1415-1422.

27. Thompson KH and Godin DV. Micronutrient and antioxidant in the progression of diabetes. Nutr. Res. 1995; 15:1377-1410.

28. Kocak G, Aktan F, Canbolat O, Ozogul C, Elbeg S, Yildizoglu-Ari N, Karasu C. Alpha-lipoic acid treatment ameliorates metabolic parameters, blood pressure, vascular reactivity and morphology of vessel already damaged by streptozotocin -diabetic rats. Diab. Nutr. Metab. 2000; 13:308-318.

29. Blasiak J, Arabski M, Krupa R, Wozniak K, Zadrozny M, Kaszniski J, Zurawska, M, Drzwoski J. DNA damage and repair in type 2 diabetes mellitus. Mutation Research. 2004; 554:297-304.

30. Imaeda A, Tanigawa T, Aoki T, Kondo Y, Nakamura N, Yoshikawa T, Antioxidative effect of fluvastatin and its metabolites against DNA damage in streptozotocin mammalian cultured cells. Free Radical Research. 2001;35:789-801.

31. Claycombe KJ, and Meydani SN. Vitamin E and genome stability. Mutation Research. 2001; 475:37-44.

32. Royack GA, Nguen MP, Tong DC, Poot M, Oda D. Respond of human oral epithelial cells to oxidative damage and the effect of vitamin E. 2000; 369(1):37-41.

33. Slamenova D, Horvathova E, Kosikova B, Ruzekova L, Labaj J. Detection of lignin biopolymer -and Vitamin E stimulated reduction of DNA strand breaks in H2O2-and MNNG -treated mammalian cells by comet assay. Nutrition Cancer. 1999; 33(1): 88-94.

34. Konopacka M, Widel M, Rzeszowska-Wolny J. Modifying effect of vitamin C, E and beta-carotene against gammaray-induced DNA damage in mouse cells. Mutation Research. 1998; 417(2/3):85-94

35. Sardas S, Yilmaz M, Oztok U, Cakir N, Karakaya AE. Assesment of DNA stand breakage by comet assay in diabetic  patients and the role of antioxidant supplement. Mutation Research 2001; 490:123-129.

36. Suzuki YJ, Tsuchiya M, Wassall SR. Structural and dynamic membrane properties of (-tocopherol and) tocotrienol: implication to the molecular mechanism of their antioxidant potency. Biochemistry. 1993; 32:10692-10699.
citation: Siti Balkis Budin, and Nor Fadilah Rajab, and Khairol Osman, and Abdul Gapor Mat Top , and Wan Nazaimoon Wan Mohamud, and Mokhtar Abu Bakar, and Jamaludin Mohamed, (2006) Effects of Palm Vitamin E Against Oxidative Damage in Streptozotocin-induced Diabetic Rats. Malaysian Journal of Biochemistry and Molecular Biology, 13 (1). pp. 11-17. ISSN ISSN 1511-2616