creators_name: Yong, Wilson, creators_name: Janna Ong Abdullah, creators_name: Maziah Mahmood, type: article datestamp: 2008-07-01 14:30:21 lastmod: 2008-07-01 14:30:21 metadata_visibility: show corp_creators: Universiti Putra Malaysia. Faculty of Biotechnology and Biomolecular Sciences. Dept. of Microbiology corp_creators: Universiti Putra Malaysia. Faculty of Biotechnology and Biomolecular Sciences. Dept. of Biochemistry corp_creators: Universiti Putra Malaysia. Faculty of Biotechnology and Biomolecular Sciences. Dept. of Microbiology title: Minimal Inhibitory Concentrations of Kanamycin on Melastoma malabathricum and Tibouchina semidecandra ispublished: pub subjects: Q subjects: R full_text_status: none keywords: Melastoma malabathricum, Tibouchina semidecandra, kanamycin, inhibitory, surviving, regeneration note: The authors wish to thank The Ministry of Science, Technology and Environmental for funding the research through IRPA Project (09-02-04-0548-EA001). Yong W. is a recipient of the National Science Fellowship abstract: Minimal inhibitory concentrations of kanamycin on shoots and nodes of both Melastoma malabathricum and Tibouchina semidecandra were assessed. Results were based on the percentage of surviving and regeneration of explants after four weeks of kanamycin treatments. The percentages of surviving and regeneration dropped as the kanamycin concentration increased until the optimal concentrations were reached to inhibit the regeneration or killed the tissues. Results showed that kanamycin at 500 and 400 mg/L were sufficient to inhibit the regeneration of Melastoma malabathricum shoots and nodes respectively, whereas 400 and 300 mg/L were inhibitory to the shoots and nodes of Tibouchina semidecandra, respectively. date: 2006 date_type: published publication: Malaysian Journal of Biochemistry and Molecular Biology volume: 13 number: 1 publisher: Malaysian Society for Biochemistry and Molecular Biology pagerange: 27-31 refereed: TRUE issn: ISSN 1511-2616 official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=590 referencetext: 1. Anshulika L, Chawla HS. Studies on factors involved in transfer of foreign gene by pipetting Agrobacterium into spikelets of wheat. Indian Journal of Genetic 2001; 61:298-300. 2. Franche C, Diouf D, Le QV, Bogusz D, N’Diaye A, Gherbi H, Gobe C, Duhoux E. Genetic transformation of the actinorhizal tree Allocasuarina verticillata by Agrobacterium tumefaciens. The Plant Journal 1997; 11: 897-904. 3. Lal R, Lal S. Crop Improvement Utilizing Biotechnology. Bota Raton, Florida: CRC Press. 1990. 4. Lindsey K, Jones MGK. Plant Biotechnology in Agriculture. Milton Keynes: Open University Press. 1989. 5. Murashige T, Skoog F. A revised medium for rapid growth and bioassays for tobacco tissue culture. Physiologia Plantarum 1962; 15: 473-497. 6. Nap JP, Bijvoet J, Stiekema WJ. Biosafety of kanamycin resistant transgenic plants. Transgenic Research 1992; 1:239-249. 7. Reynaerts A, De Block M, Hernalsteens JP, Van Montagu M. Selectable and screenable markers. In: Gelvin SB, Schilperoort RA, Verma DPS, eds. Plant Molecular Biology Manual. pp. A9: 1-16. Dordrecht: Kluwer Academic Publishers. 1988. 8. Seabra RC, Pais MS. Genetic transformation of European chestnut. Plant Cell Reports 1998; 17: 177-182. 9. Shaw CH. Plant Molecular Biology: A Practical Approach. Oxford, Washington DC: IRL Press. 1988. 10. Xiang CB, Han P, Oliver DJ. In solium selection for Arabidopsis transformants resistant to kanamycin. Plant Molecular Biology Reporter 1999; 17: 59-65. 11. Yang J, Lee HJ, Shin DH, Oh SK, Seon JH, Paek KY, Han KH. Genetic transformation of Cymbidium orchid by particle bombardment. Plant Cell Reports 1999; 18: 978-984. 12. Zhang J, Tiwari VK, Golds TJ, Blackhall NW, Cocking EC, Mulligan JB, Davey MR. Parameters influencing transient and stable transformation of barley (Hordeum vulgare L.) protoplasts. Plant Cell, Tissue and Organ Culture 1995; 41: 125-138. citation: Yong, Wilson, and Janna Ong Abdullah, and Maziah Mahmood, (2006) Minimal Inhibitory Concentrations of Kanamycin on Melastoma malabathricum and Tibouchina semidecandra. Malaysian Journal of Biochemistry and Molecular Biology, 13 (1). pp. 27-31. ISSN ISSN 1511-2616