2247 11 archive 2 disk0/00/00/22/47 2008-07-01 14:27:51 2008-07-01 14:27:51 2008-07-01 14:27:51 article show Suhana Md. Saad Yasmin Anum Mohd Yusof Wan Ngah W.Z. Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry pub Q R none Chlorella vulgaris, apoptosis, antioxidant, oxidative damage This research was supported by RM-8 IRPA grant 06- 02-02-0023-PR 0008/09-08 from Ministry of Science, Technology and Innovation (MOSTI). The Chlorella vulgaris from Japan was a kind gift from Prof. Takeshi Hasegawa from Research Laboratories, Chlorella Industry, Chikugo City, Fukuoko, Japan. Chlorella vulgaris (CV), a unicellular green algae, has been shown to have many positive effects in human and animal studies in Japan and in the United States. In this study, we cultured CV in the laboratory to study its anti-carcinogenic properties in liver cancer cell line, HepG2. At the same time we compared the anti-carcinogenic properties of locally produced CV with that from Japan. Liver cancer cell line, HepG2 and normal liver cell line, WRL68, were treated with hot water extract of CV cultured in the laboratory (CVL) and with hot water extract of CV from Japan (CVJ). Results from the BrdU assay showed that CVJ increased proliferation of HepG2 cells initially and reduced later at higher concentration of CVJ extract. However, HepG2 cell line treated with hot water extract of CVL showed significant decrease in proliferation rate ; with a 50% reduction at 1600 μg/ml of CV extract (IC 50 of CVL for HepG2). Proliferation of WRL68 decreased with a 50% reduction at 1700 μg/ml of CV extract (IC 50 of CV for WRL68). 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