creators_name: Gam,  Lay Harn,  
creators_name: Aishah Latiff,  
type: article
datestamp: 2008-07-01 15:52:59
lastmod: 2008-07-01 15:52:59
metadata_visibility: show
corp_creators: Universiti Sains Malaysia. School of Pharmaceutical Sciences
corp_creators: Universiti Sains Malaysia. Doping Control Centre
title: Identification and Confirmatory Method for Human Chorionic Gonadotropin (hCG) using HPLC/MS/MS Analysis
ispublished: pub
subjects: Q
subjects: R
full_text_status: none
keywords: Human Chorionic Gonadotropin, Tandem mass spectrometry
abstract: As a consequence of the abused of human chorionic gonadotropin αhCG) by male athletes, a reliable detection method for βhCG is highly desired in sport testing. hCG is misused by male athletes to stimulate endogenous secretion of testosterone, which led to the ban of the hormone by the International Olympic Committee (IOC) in 1987. The present study demonstrated mass spectrometric characterization of hCG. The feasibility of tryptic peptides of hCG as marker were investigated. The fingerprints of peptides which reveal in their amino acid sequences were obtained to provide an unequivocal identification and confirmation of hCG. It is through this technique that 63% and 91% respectively of the amino acid sequence of alpha- and beta-subunit of hCG were determined.
date: 2005
date_type: published
publication: Malaysian Journal of Biochemistry and Molecular Biology
volume: 12
number: 1
publisher: Malaysian Society for Biochemistry and Molecular Biology
pagerange: 25-30
refereed: TRUE
issn: ISSN 1511-2616
official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=608
referencetext: 1. Ross GT, Cargille CM, Lipsett MB, Rayford RL, Narshall JR, Strott CA, Rodbard D. Pituitary and gonadal hormones in women during spontaneous and induced ovulatory cycles. Rec Progr Horm Res 1970; 26:1-62.

2. Vande Wiele RL, Bogumil J, Dyrenfurth I, Ferin M, Jewelewicz R, Warren M, Rizkallah T, Mikhail G. Mechanisms regulating the menstrual cycle in women. Rec Progr Horm Res 1970; 26:63-103.

3. Chen HC, Hodgen GD, Matsuura B, Lin LJ, Gross E, Reichert LE, Birken S, Canfield RE. Evidence for gonadotropin from nonpregnant subjects that has physical, immunologic and biologic similarities to hCG. Proc Natl Acad Sci USA. 1976; 72:2885-2890.

4. Canfield RE, Birken S, Morse JH, Morgan FJ. Human Chorionic Gonadotroin. In: Peptide Hormones. Parsons JA ed. The Macmillan press LTP. Pp 299-385. 1976.

5. Kessler MJ, Reddy MS, Shah RH, Bahl OP. Structure of N-glycosidic carbohydrate units of hCG. J Biol Chem 1979; 254: 7901-7903.

6. Kessler MJ, Mise T, Chai RD, Bahl OP.. Structure and location of O-glycosidic carbohydrate units of hCG. J Biol Chem 1979; 254: 7904-7908.

7. Cowan DA, Kicman AT, Walker CJ, Wheeler MJ.. Effect of administration of hCG on criteria used to assess testosterone administration in athletes. J Endocrinol 1991; 131: 147-154.

8. Gam LH, Aishah L. High throughput screening for urinary human chorionic gonadotropin using ELISA method. J Bioscience 2002; 2 (13) 95-101

9. Hussa RO, Martha LR, Paula GS. Discondant hCG results: Causes and solutions. Obstet Gynecol 1985; 65:211-219. 10. Gam LH, Aishah L. Identification and confirmation of Urinary Human Chorionic Gonadotropin using In-gel digestion and HPLC/MS/MS analysis. J Bioscience 2002;1 (13) 27-34.

11. Liu CL, Bowers LD. Immunoaffinity trapping of urinary human chorionic gonadotropin as its high-performance liquid chromatographic-mass spectrometric confirmation. J Chromatogr B 1996; 687: 213-220.

12. Laidler P, Cowan DA, Hider RC, Kicman AT. Qualitative analysis of hCG for confirmatory purposes. In Recent Advances in Doping Analysis: Proceedings of the 14th Cologne Workshop on Dope Analysis. Schanzer W, Geyer H, Gotzmann A, Mareck-Engelke U. eds. pp 285-304.

13. Morgan FJ, Birken S, Canfield RE. Human chorionic gonadotropin: a proposal for the amino acid sequence. Mol Cell Biochem 1973; 2:97-99.

14. Sarla P, Kui S, Sathyamangalam VB, Bahl OP. Mutants of human choriogonadotropin lacking N-glycosyl chains in the alpha-subunit. I. Mechanism for the differential action of the N-linked carbohydrates. Biochem 1997; 35:12355-12363.
citation: Gam, Lay Harn, and Aishah Latiff, (2005) Identification and Confirmatory Method for Human Chorionic Gonadotropin (hCG) using HPLC/MS/MS Analysis. Malaysian Journal of Biochemistry and Molecular Biology, 12 (1). pp. 25-30. ISSN ISSN 1511-2616