creators_name: Mhd. Nasir Mohd Desa,  
creators_name: Thong, Kwai Lin,  
creators_name: Parasakhti Navaratnam,  
creators_id: 
creators_id: thongkl@um.edu.my
creators_id: 
type: article
datestamp: 2008-07-03 06:46:51
lastmod: 2008-07-03 06:46:51
metadata_visibility: show
corp_creators: Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences
corp_creators: University of Malaya. Institute of Biological Sciences
corp_creators: University of Malaya. Faculty of Medicine. Dept. of Microbiology
title: A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility
ispublished: pub
subjects: Q
subjects: R
full_text_status: none
keywords: Streptococcus pneumoniae, Penicillin, Erythromycin, MIC
note: This study was supported by the University of Malaya VoteF grant F0100/2001D.
abstract: The correlation of pbp 2b restriction fragment length polymorphism (RFLP) patterns of fifty clinical isolates of Malaysian Streptococcus pneumoniae, as well as the distribution of determinant genes for erythromycin resistance; mefE and ermB, with the respective minimum inhibitory concentration (MIC) values for penicillin and erythromycin (determined by agar dilution method) were examined. Strains with penicillin MIC of ≥ 0.5 μg/ml had unique pbp 2b RFLP patterns that were absent in the penicillinsusceptible strains. The prevalence of ermB, either alone or together with mefE, was frequently observed in strains with higher MIC values (> 8 μg/ml) whereas mefE alone was observed in strains with lower MIC values (1 μg/ml - 4 μg/ml). This showed that the pbp 2b RFLP patterns and the distribution of mefE and ermB genes followed certain patterns in relation to the susceptibility. This correlation may thus be used as a diagnostic criterion for molecular detection of resistance.
date: 2005
date_type: published
publication: Malaysian Journal of Biochemistry and Molecular Biology
volume: 12
number: 1
publisher: Malaysian Society for Biochemistry and Molecular Biology
pagerange: 55-57
refereed: TRUE
issn: ISSN 1511-2616
official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=612
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citation: Mhd. Nasir Mohd Desa, and Thong, Kwai Lin, and Parasakhti Navaratnam, (2005) A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility. Malaysian Journal of Biochemistry and Molecular Biology, 12 (1). pp. 55-57. ISSN ISSN 1511-2616