@article{myais2264,
          volume = {12},
          number = {1},
           month = {["lib/utils:month\verb1_12264" not defined]},
          author = {  Nik Marzuki Sidik and   Shaiful Adzni Sharifuddin and   Sahidan Senafi},
            note = {This study was funded by an IRPA grant 09-02-02-0108-EA260, Ministry of Science, Technology and Innovation, Malaysia.},
           title = {Cloning of a Partial $\beta$-glucosidase Gene From Aspergillus terreus SUK-1},
       publisher = {Malaysian Society for Biochemistry and Molecular Biology},
            year = {2005},
         journal = {Malaysian Journal of Biochemistry and Molecular Biology},
           pages = {61--63},
        keywords = {Aspergillus terreus SUK-1, cellulase, ?-glucosidase, PCR},
             url = {http://myais.fsktm.um.edu.my/2264/},
        abstract = {The breakdown of cellulose to glucose is catalysed by a complex cellulase enzyme which is comprised of three classes of enzymes; endoglucanase, exoglucanase and ?-glucosidase. Many studies have been done on cellulase and several genes encoding for ?-glucosidase have been reported. Here we describe the cloning and sequence analysis of partial putative ?-glucosidase gene from Aspergillus terreus SUK-1. Its potential to produce cellulase enzyme has been studied and reported. A pair of specific primer was designed from the conserved regions of several fungal sequences taken out from the GenBank. PCR amplification has successfully amplified a DNA fragment of approximately 233 bp and this fragment was cloned (designated as SA3). The sequence analysis with BLAST showed high similarity towards ?-glucosidase gene and protein. 80% and 79% homology were observed between SA3 nucleotide sequence and ?-glucosidase gene of A. niger and both A. kawachii and A. aculeatus. Whereas, amino acid sequence of SA3 showed 83% homology towards ?-glucosidase of A. niger, A. kawachii and A. aculeatus.}
}