title: Cloning of a Partial β-glucosidase Gene From Aspergillus terreus SUK-1
creator: Nik Marzuki Sidik,  
creator: Shaiful Adzni Sharifuddin,  
creator: Sahidan Senafi,  
subject: Q Science, Computer Science
subject: R Medicine, Dentistry, Pharmacy, Nursing
description: The breakdown of cellulose to glucose is catalysed by a complex cellulase enzyme which is comprised of three classes of enzymes; endoglucanase, exoglucanase and β-glucosidase. Many studies have been done on cellulase and several genes encoding for β-glucosidase have been reported. Here we describe the cloning and sequence analysis of partial putative β-glucosidase gene from Aspergillus terreus SUK-1. Its potential to produce cellulase enzyme has been studied and reported. A pair of specific primer was designed from the conserved regions of several fungal sequences taken out from the GenBank. PCR amplification has successfully amplified a DNA fragment of approximately 233 bp and this fragment was cloned (designated as SA3). The sequence analysis with BLAST showed high similarity towards β-glucosidase gene and protein. 80% and 79% homology were observed between SA3 nucleotide sequence and β-glucosidase gene of A. niger and both A. kawachii and A. aculeatus. Whereas, amino acid sequence of SA3 showed 83% homology towards β-glucosidase of A. niger, A. kawachii and A. aculeatus.
publisher: Malaysian Society for Biochemistry and Molecular Biology
date: 2005
type: Journal
type: PeerReviewed
relation: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=614
identifier: Nik Marzuki Sidik, and Shaiful Adzni Sharifuddin, and Sahidan Senafi, (2005) Cloning of a Partial β-glucosidase Gene From Aspergillus terreus SUK-1. Malaysian Journal of Biochemistry and Molecular Biology, 12 (1). pp. 61-63. ISSN ISSN 1511-2616
relation: http://myais.fsktm.um.edu.my/2264/