%0 Journal Article
%@ ISSN 1511-2616
%A Nik Marzuki Sidik,  
%A Shaiful Adzni Sharifuddin,  
%A Sahidan Senafi,  
%D 2005
%F myais:2264
%I Malaysian Society for Biochemistry and Molecular Biology
%J Malaysian Journal of Biochemistry and Molecular Biology
%K Aspergillus terreus SUK-1, cellulase, ?-glucosidase, PCR
%N 1
%P 61-63
%T Cloning of a Partial ?-glucosidase Gene From Aspergillus terreus SUK-1
%U http://myais.fsktm.um.edu.my/2264/
%V 12
%X The breakdown of cellulose to glucose is catalysed by a complex cellulase enzyme which is comprised of three classes of enzymes; endoglucanase, exoglucanase and ?-glucosidase. Many studies have been done on cellulase and several genes encoding for ?-glucosidase have been reported. Here we describe the cloning and sequence analysis of partial putative ?-glucosidase gene from Aspergillus terreus SUK-1. Its potential to produce cellulase enzyme has been studied and reported. A pair of specific primer was designed from the conserved regions of several fungal sequences taken out from the GenBank. PCR amplification has successfully amplified a DNA fragment of approximately 233 bp and this fragment was cloned (designated as SA3). The sequence analysis with BLAST showed high similarity towards ?-glucosidase gene and protein. 80% and 79% homology were observed between SA3 nucleotide sequence and ?-glucosidase gene of A. niger and both A. kawachii and A. aculeatus. Whereas, amino acid sequence of SA3 showed 83% homology towards ?-glucosidase of A. niger, A. kawachii and A. aculeatus.
%Z This study was funded by an IRPA grant 09-02-02-0108-EA260, Ministry of Science, Technology and Innovation, Malaysia.