TY  - JOUR
ID  - myais2264
UR  - http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=614
IS  - 1
A1  - Nik Marzuki Sidik,  
A1  - Shaiful Adzni Sharifuddin,  
A1  - Sahidan Senafi,  
N2  - The breakdown of cellulose to glucose is catalysed by a complex cellulase enzyme which is comprised of three classes of enzymes; endoglucanase, exoglucanase and ?-glucosidase. Many studies have been done on cellulase and several genes encoding for ?-glucosidase have been reported. Here we describe the cloning and sequence analysis of partial putative ?-glucosidase gene from Aspergillus terreus SUK-1. Its potential to produce cellulase enzyme has been studied and reported. A pair of specific primer was designed from the conserved regions of several fungal sequences taken out from the GenBank. PCR amplification has successfully amplified a DNA fragment of approximately 233 bp and this fragment was cloned (designated as SA3). The sequence analysis with BLAST showed high similarity towards ?-glucosidase gene and protein. 80% and 79% homology were observed between SA3 nucleotide sequence and ?-glucosidase gene of A. niger and both A. kawachii and A. aculeatus. Whereas, amino acid sequence of SA3 showed 83% homology towards ?-glucosidase of A. niger, A. kawachii and A. aculeatus.
VL  - 12
TI  - Cloning of a Partial ?-glucosidase Gene From Aspergillus terreus SUK-1
AV  - none
EP  - 63
N1  - This study was funded by an IRPA grant 09-02-02-0108-EA260, Ministry of Science, Technology and Innovation, Malaysia.
Y1  - 2005///
PB  - Malaysian Society for Biochemistry and Molecular Biology
JF  - Malaysian Journal of Biochemistry and Molecular Biology
KW  - Aspergillus terreus SUK-1
KW  -  cellulase
KW  -  ?-glucosidase
KW  -  PCR
SN  - ISSN 1511-2616
SP  - 61
ER  -