creators_name: Nik Marzuki Sidik,  
creators_name: Shaiful Adzni Sharifuddin,  
creators_name: Sahidan Senafi,  
creators_id: nms@pkrisc.cc.ukm.my
creators_id: 
creators_id: 
type: article
datestamp: 2008-07-03 06:42:41
lastmod: 2008-07-03 06:42:41
metadata_visibility: show
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Bioscience and Biotechnology
corp_creators: Malaysian Argricultural Research and Development Institute. Biotechnology Center,
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Bioscience and Biotechnology
title: Cloning of a Partial β-glucosidase Gene From Aspergillus terreus SUK-1
ispublished: pub
subjects: Q
subjects: R
full_text_status: none
keywords: Aspergillus terreus SUK-1, cellulase, β-glucosidase, PCR
note: This study was funded by an IRPA grant 09-02-02-0108-EA260, Ministry of Science, Technology and Innovation, Malaysia.
abstract: The breakdown of cellulose to glucose is catalysed by a complex cellulase enzyme which is comprised of three classes of enzymes; endoglucanase, exoglucanase and β-glucosidase. Many studies have been done on cellulase and several genes encoding for β-glucosidase have been reported. Here we describe the cloning and sequence analysis of partial putative β-glucosidase gene from Aspergillus terreus SUK-1. Its potential to produce cellulase enzyme has been studied and reported. A pair of specific primer was designed from the conserved regions of several fungal sequences taken out from the GenBank. PCR amplification has successfully amplified a DNA fragment of approximately 233 bp and this fragment was cloned (designated as SA3). The sequence analysis with BLAST showed high similarity towards β-glucosidase gene and protein. 80% and 79% homology were observed between SA3 nucleotide sequence and β-glucosidase gene of A. niger and both A. kawachii and A. aculeatus. Whereas, amino acid sequence of SA3 showed 83% homology towards β-glucosidase of A. niger, A. kawachii and A. aculeatus.
date: 2005
date_type: published
publication: Malaysian Journal of Biochemistry and Molecular Biology
volume: 12
number: 1
publisher: Malaysian Society for Biochemistry and Molecular Biology
pagerange: 61-63
refereed: TRUE
issn: ISSN 1511-2616
official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=614
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citation: Nik Marzuki Sidik, and Shaiful Adzni Sharifuddin, and Sahidan Senafi, (2005) Cloning of a Partial β-glucosidase Gene From Aspergillus terreus SUK-1. Malaysian Journal of Biochemistry and Molecular Biology, 12 (1). pp. 61-63. ISSN ISSN 1511-2616