http://myais.fsktm.um.edu.my/2266/ Functions of Human GM-CSF α-Subunit in Mediating Self-Renewal or Differentiation on FDCP-Mix Cells Shahrul Hisham Zainal Ariffin, Rohaya Megat Abdul Wahab, Zaidah Zainal Ariffin, Roslina Samsudin, Siti Fatimah Mohd Noor, Whetton, A.D., Q Science, Computer Science R Medicine, Dentistry, Pharmacy, Nursing Activation of human GM-CSF receptor ectopically expressed in polyclonal murine FDCP-Mix multipotent cells, stimulate selfrenewal or myeloid differentiation. Bioinformatic analysis revealed a potentially important tripeptide region in the α-subunits cytosolic domain. Mutation of hGM-CSF receptor α-subunit (hGM-CSFRα) involving the respective tripeptides altered the receptor function compared to the wild type receptor. The mutated hGM-CSFRα clonal cells signaled for self-renewal whilst the wild type receptor produce cell differentiations in the presence of hGM-CSF cytokines. These results showed that cytosolic region of the α-subunit was critically important for developmental decision in multipotent cell, indicating that signaling specificity for cell fate resides in the receptor’s structure. Malaysian Society for Biochemistry and Molecular Biology 2005 Journal PeerReviewed http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=616 Shahrul Hisham Zainal Ariffin, and Rohaya Megat Abdul Wahab, and Zaidah Zainal Ariffin, and Roslina Samsudin, and Siti Fatimah Mohd Noor, and Whetton, A.D., (2005) Functions of Human GM-CSF α-Subunit in Mediating Self-Renewal or Differentiation on FDCP-Mix Cells. Malaysian Journal of Biochemistry and Molecular Biology, 11 (1). pp. 6-12. ISSN ISSN 1511-2616 http://myais.fsktm.um.edu.my/2266/