%0 Journal Article
%@ ISSN 1511-2616
%A Shahrul Hisham Zainal Ariffin,  
%A Rohaya Megat Abdul Wahab,  
%A Zaidah Zainal Ariffin,  
%A Roslina Samsudin,  
%A Siti Fatimah Mohd Noor,  
%A Whetton, A.D.,  
%D 2005
%F myais:2266
%I Malaysian Society for Biochemistry and Molecular Biology
%J Malaysian Journal of Biochemistry and Molecular Biology
%K GM-CSF receptor, multipotent cells, differentiation
%N 1
%P 6-12
%T Functions of Human GM-CSF ?-Subunit in Mediating Self-Renewal or Differentiation on FDCP-Mix Cells
%U http://myais.fsktm.um.edu.my/2266/
%V 11
%X Activation of human GM-CSF receptor ectopically expressed in polyclonal murine FDCP-Mix multipotent cells, stimulate selfrenewal or myeloid differentiation. Bioinformatic analysis revealed a potentially important tripeptide region in the ?-subunits cytosolic domain. Mutation of hGM-CSF receptor ?-subunit (hGM-CSFR?) involving the respective tripeptides altered the receptor function compared to the wild type receptor. The mutated hGM-CSFR? clonal cells signaled for self-renewal whilst the wild type receptor produce cell differentiations in the presence of hGM-CSF cytokines. These results showed that cytosolic region of the ?-subunit was critically important for developmental decision in multipotent cell, indicating that signaling specificity for cell fate resides in the receptor?s structure.