Functions of Human GM-CSF α-Subunit in Mediating Self-Renewal or Differentiation on FDCP-Mix Cells Shahrul Hisham Zainal Ariffinauthor Rohaya Megat Abdul Wahabauthor Zaidah Zainal Ariffinauthor Roslina Samsudinauthor Siti Fatimah Mohd Noorauthor Whetton, A.D.authorActivation of human GM-CSF receptor ectopically expressed in polyclonal murine FDCP-Mix multipotent cells, stimulate selfrenewal or myeloid differentiation. Bioinformatic analysis revealed a potentially important tripeptide region in the α-subunits cytosolic domain. Mutation of hGM-CSF receptor α-subunit (hGM-CSFRα) involving the respective tripeptides altered the receptor function compared to the wild type receptor. The mutated hGM-CSFRα clonal cells signaled for self-renewal whilst the wild type receptor produce cell differentiations in the presence of hGM-CSF cytokines. These results showed that cytosolic region of the α-subunit was critically important for developmental decision in multipotent cell, indicating that signaling specificity for cell fate resides in the receptor’s structure.Q Science, Computer ScienceR Medicine, Dentistry, Pharmacy, Nursing2005Malaysian Society for Biochemistry and Molecular BiologyJournal