%A   Shahrul Hisham Zainal Ariffin
%A   Rohaya Megat Abdul Wahab
%A   Zaidah Zainal Ariffin
%A   Roslina Samsudin
%A   Siti Fatimah Mohd Noor
%A   Whetton, A.D.
%J Malaysian Journal of Biochemistry and Molecular Biology
%T Functions of Human GM-CSF ?-Subunit in Mediating Self-Renewal or Differentiation on FDCP-Mix Cells
%X Activation of human GM-CSF receptor ectopically expressed in polyclonal murine FDCP-Mix multipotent cells, stimulate selfrenewal or myeloid differentiation. Bioinformatic analysis revealed a potentially important tripeptide region in the ?-subunits cytosolic domain. Mutation of hGM-CSF receptor ?-subunit (hGM-CSFR?) involving the respective tripeptides altered the receptor function compared to the wild type receptor. The mutated hGM-CSFR? clonal cells signaled for self-renewal whilst the wild type receptor produce cell differentiations in the presence of hGM-CSF cytokines. These results showed that cytosolic region of the ?-subunit was critically important for developmental decision in multipotent cell, indicating that signaling specificity for cell fate resides in the receptor?s structure.
%N 1
%K GM-CSF receptor, multipotent cells, differentiation
%P 6-12
%V 11
%D 2005
%I Malaysian Society for Biochemistry and Molecular Biology
%L myais2266