creators_name: Nathan, Sheila,  
creators_name: Yap, Thai Leong,  
creators_name: Mohd Shazrul Fazry Sa’ariwijaya,  
type: article
datestamp: 2008-07-03 06:23:21
lastmod: 2008-07-03 06:23:21
metadata_visibility: show
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Bioscience and Biotechnology
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Bioscience and Biotechnology
corp_creators: Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Bioscience and Biotechnology
title: Burkholderia pseudomallei Secretes Metallo- and Serine Proteases in Culture
ispublished: pub
subjects: Q
subjects: R
full_text_status: none
keywords: B. pseudomallei, serine protease, metalloprotease, protease inhibitors
abstract: Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis in humans and livestock in Southeast Asia and Northern Australia. Several virulence factors including an extracellular protease have been implicated in the pathogenesis of melioidosis. In the study reported here, our aim was to monitor the optimum length of time for protease production and determine the class of extracellular protease over seven consecutive days of in vitro culture. All the harvested culture filtrates of B. pseudomallei were ammonium sulfate precipitated, dialysed and lyophilized prior to DEAE-Sephacel anion exchange chromatography. The purified protease was characterized using serine, cysteine, aspartate and metalloprotease inhibitors. Protease characterization by SDS-PAGE and zymography identified a protein of 37 kDa. Protease activity was evaluated both in culture filtrates and partially purified protease by azocasein hydrolysis at 405 nm absorbency. Based on the data, we conclude that B. pseudomallei in culture secretes a metallo and a serine protease which could be involved in disease manifestation.
date: 2005
date_type: published
publication: Malaysian Journal of Biochemistry and Molecular Biology
volume: 11
number: 1
publisher: Malaysian Society for Biochemistry and Molecular Biology
pagerange: 13-19
refereed: TRUE
issn: ISSN 1511-2616
official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=617
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citation: Nathan, Sheila, and Yap, Thai Leong, and Mohd Shazrul Fazry Sa’ariwijaya, (2005) Burkholderia pseudomallei Secretes Metallo- and Serine Proteases in Culture. Malaysian Journal of Biochemistry and Molecular Biology, 11 (1). pp. 13-19. ISSN ISSN 1511-2616