creators_name: Lee, Kheng Ji, creators_name: Eshaghi, Majid, creators_name: Khatijah Yusof, creators_name: Tan, Wen Siang, type: article datestamp: 2008-07-03 06:10:19 lastmod: 2008-07-03 06:10:19 metadata_visibility: show corp_creators: Universiti Putra Malaysia. Faculty of Biotechnology and Biomolecular Sciences. Dept. of Microbiology corp_creators: Universiti Putra Malaysia. Faculty of Biotechnology and Biomolecular Sciences. Dept. of Microbiology corp_creators: Universiti Putra Malaysia. Institute of Bioscience corp_creators: Universiti Putra Malaysia. Institute of Bioscience title: Inhibition of Newcastle Disease Virus Propagation In Vero Cells By a Recombinant Bacteriophage And A Synthetic Peptide ispublished: pub subjects: Q subjects: R full_text_status: none keywords: Filamentous phage, peptide, inhibitory activities, cell culture note: This study was supported by the IRPA grant 01-02-04-003 BTK/ER/006 from the Ministry of Science, Technology and Innovation, Malaysia. abstract: A recombinant filamentous phage bearing the peptide sequence CTLTTKLYC has been shown to interact tightly with Newcastle disease virus (NDV). The peptide, either alone or in the phage associated form, inhibited the hemolytic activity of the virus and also its propagation in embryonated chicken eggs. Here, we demonstrate that phage bearing the peptide sequence CTLTTKLYC and a synthetic peptide derived from the sequence inhibit the infection of Vero cells by NDV. date: 2005 date_type: published publication: Malaysian Journal of Biochemistry and Molecular Biology volume: 11 number: 1 publisher: Malaysian Society for Biochemistry and Molecular Biology pagerange: 20-23 refereed: TRUE issn: ISSN 1511-2616 official_url: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=618 referencetext: 1. Rima BK, Alexander DJ, Billeter MA, Collins PL, Kingsbury DW, Lipkind MA, Nagai Y, Oervell C, Pringle CR, ter Meulen V. The Paramyxoviridae. In: Murphy FA, Fouquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summer MD eds. Virus Taxonomy, Classification and Nomenclature of Viruses. Sixth report of the International Committee on Taxonomy of Viruses. New York: Springer Verlag. 1995 2. Mayo MA. A summary of taxonomy change recently approved by ICTV. Arch Virology 2002; 147: 1656-1676. 3. Nagai Y, Klenk HD, Rott R. Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 1976; 72: 494-508. 4. Yusoff K, Tan WS. Newcastle disease virus: macromolecules and opportunities. Avian Path 2001; 30: 439-455. 5. Phillips RJ, Samson ACR, Emmerson PT. Nucloetide sequence of the 5í-terminus of Newcastle disease virus and assembly of the complete genomic sequence: agreement with the ërule of sixí. Arch Virol 1998; 143: 1993-2002. 6. Sinkovics, JG, Horvath JC. Newcastle disease virus (NDV): a brief history of its oncolytic strains. J Clin Virol 2000; 16: 1-15. 7. Scheid A, Choppin PW. The haemagglutinin and References neuraminidase protein of a paramyxovirus: interaction with neuraminic acid in affinity chromatography. Virology 1974; 62: 125-133. 8. Mirza AM, Deng R, Iorio RM. Site-directed mutagenesis of a conserved hexapeptide in the paramyxovirus haemagglutinin-neuraminidase glycoprotein: effects on antigenic structure and function. J Virol 1994; 68: 5093-5099. 9. Ramanujam P, Tan WS, Nathan S, Yusoff K. Novel peptides that inhibit the propagation of Newcastle disease virus. Arch Virol 2002; 147: 981-993. 10. Yusoff K., Tan W.S., Lau C.H., Ng B,K., Ibrahim L. Sequence of the haemagglutinin-neuraminidase gene of the Newcastle disease virus oral vaccine strain V4 (UPM). Avian Pathol 1996; 25: 837-844. 11. Smith GP, Scott JK. Libraries of peptides and proteins displayed on filamentous phage. Meth Enzymol 1993;217: 228-257. 12. Sambrook J, Fritsch, EF, Maniatis, T. Molecular cloning: A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratories Press 1989. 13. Alexander DJ. Newcastle Disease Diagnosis. In: Alexander DJ ed. Newcastle Disease. London: Kluwer Academic.. 1988. citation: Lee, Kheng Ji, and Eshaghi, Majid, and Khatijah Yusof, and Tan, Wen Siang, (2005) Inhibition of Newcastle Disease Virus Propagation In Vero Cells By a Recombinant Bacteriophage And A Synthetic Peptide. Malaysian Journal of Biochemistry and Molecular Biology, 11 (1). pp. 20-23. ISSN ISSN 1511-2616